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Rose plant is important horticulturally as a cut flower and as a source of rose oil, with
high domestic and overseas demand. New varieties are constantly being developed with
better qualities. Conventionally, rose is propagated through cuttings, layering, bud grafting
and occasionally through seed, depending on the varieties (Pal, 1966). Most of the cultivars
are heterozygous and do not breed true to type. Tissue culture technique is being applied
of late to ensure homozygous genetic status and large-scale quick multiplication throughout
the year, irrespective of the seasonal variation.
In the present investigation, Rosa
hybrida cv. King's Ransom with dense foliage
and demanding floral attributes was selected to study the in vitro response to axillary shoot generation and obtain maximum number of shoots from single explant for large-scale
clonal multiplication.
The plant material, Rosa hybrida cv. King's Ransom was collected from the rose beds
of the Agri-Horticulture Society of India, Calcutta. The plant was sprayed with
fungicide (Bavistin 0.5% w/v) three times at seven days interval and kept covered with a
perforated polythene bag.
The stem cutting containing 8-9 buds from the apex were excised and kept under
running water for 15 min. After washing, they were individually dissected, marked and sterilized
in HgCl2 (0.25% w/v) along with labklin (0.1% w/v) as surfactant for 10 min and washed
twice. The explants were again dissected prior to inoculation. Surface sterilization, washing
and dissection were performed under laminar airflow chamber (Klenzaids Co., Model no. 1104). |
