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There is an exigent need for development of indigenous alternative antimicrobial
molecules for the effective treatment of some serious diseases in the light of growing cases of
microbial resistance to the time-honored antibiotics. Life-saving imported allopathic drugs are
very expensive (Apisariyakul et al., 1995) and have a few side effects also (Ali-Shytayeh et al., 1998).
Though literature pertaining to the identification of medicinal plants for various
diseases and their active principles are vast, the extraction procedures are many and show the
whims and vagaries. Keeping in mind the above lacunae, the current study was undertaken
to identify the best herbal extract showing the maximum efficient antimicrobial activity
and also to standardize a suitable extracting
protocol.
Six enteric bacterial pathogens were isolated and characterized from stool samples
collected from patients with gastro intestinal disorders. The clinical isolates
were P. vulgaris, S. typhi, Citrobacter spp., E. coli, K. pneumoniae and E.
aerogenes. Fresh cultures were grown in trypticase soy broth before inoculating onto the Muller-Hinton Agar plates for
antibacterial assay.
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