The paper discusses the applications of genetic engineering protocols for efficient synthesis of high yielding varieties in tobacco species. Transgenic Nicotiana species can be synthesized using Agrobacterium tumefaciens as a vector that causes manipulation in the fundamental biological processes. Successful experimental protocol on genetic transformation is proposed to develop healthy transgenic gene expression in Nicotiana tobaccum.

Plants, especially the cereal grains, wheat, rice and corn, provide well over 80% of the total food consumed daily by humans. The demand for economically important plants is increasing due to the rapid pace of growth and development in the world. To keep abreast with the growing population, tactful management of plant growth and development is required, since the present situation, if left unmonitored, will severely place additional stress on the plant-growing capacity of the earth.

Tobacco is a tall leafy annual plant that belongs to the solanaceae or nightshade family which consists of crop plants, perennial flowering plants, poisonous weeds, various herbs, shrubs and trees. India produces several types of tobacco, which fall under two botanical species, namely, Nicotiana tabaccum and Nicotiana rustica. The quality of tobacco is greatly influenced by the soil conditions (Grappin et al., 2000). Tobacco is adapted to moderately acidic soils, with a pH ranging from 5.5 to 6.5.

Today, many agronomically and horticulturally important species are routinely transformed using this bacterium, and the list of species that is susceptible to Agrobacterium - mediated transformation seems to grow daily.