This paper investigates the type of mulberry cultivar and the enzyme taken during protoplast isolation from mesophyll cells of mulberry. Axenic shoot cultures of four mulberry cultivars (Morus indica) M5, S34, S54 and Mysore-local were cultured from nodal explants. The cultures were made at various treatment times and the levels of osmoticum for all the four cultivars. A comprehensive Mulberry Protoplast Database (MPD) was constructed from the results obtained, using query language. Moreover, since the database is user-friendly, and with the presence Graphical User Interface (GUI) menu, the user is at ease, and gets the results in a well-formatted and legible manner. Complex queries have been written in such a manner that facilitate the return of answers promptly, and without any trouble to the user. The protoplast database provides a fast, convenient and easy way to identify the yield of protoplasts and duration changes of a selected cultivar. Using the various queries of the MPD, it was concluded that protoplast yield in mulberry is dependent on the type of cultivar used and that 4-5 hours of incubation resulted in maximum protoplast yield, as compared to prolonged incubation duration in all the cultivars studied. Also, the protoplast yield was maximum in Mysore-local followed by M5, S34, and S54.
Mulberry is a perennial woody plant used mainly in the silk industry. Some mulberry varieties, namely, M5, S34, S54 and Mysore-local belonging to Morus indica species are in commercial exploitation. As they have been developed through conventional breeding techniques, they lack certain desirable traits. Due to erratic sex expression in mulberry, a non-conventional approach is needed to develop elite strains both for leaf yield and resistance factors. Attempts have been made to study protoplast isolation and purification using in vitro grown leaves of four different commercially exploited Indian mulberry varieties (M5, S34, S54 and Mysore-local), taking into consideration, the aforesaid parameters (Evans and Bravo, 1983; Katagiri, 1988, 1989; Bindroo et al., 1996; Chand et al., 1996; Tewari et al., 1996; Hassanein and Soltan, 2000; Chakravarthy et al., 2001; Kapur et al., 2001; Chakravarthy and Sadanandam, 2003; Khurana et al., 2003;).
Also, a comprehensive Mulberry Protoplast Database (MPD) of the results of this study was designed and created. This database facilitates fast access of any data required through various queries. The database is scalable and expandable, and provides a secure medium to store this critical data. Moreover, the database has a user-friendly front end, which helps the user access any data he wants from the database. |
