In self-pollinated crops hybrid breeding is difficult, because floral traits are
unfavorable for out crossing. As rice is the principal food crop of India, population explosion
stresses the need to increase the productivity of rice crop. Among the different
improved technologies to increase the productivity of rice, the exploitation of hybrid
technology appears to be promising.
However, all lines do not perform equally well and also give variable heterosis
with restoration. Thus, the characterization of cytoplasmic genic male sterile lines for
floral traits is one of the important aspects that a breeder should keep in mind while
selecting a line in hybrid breeding. Chinese rice scientists developed rice hybrids utilizing
CGMS, while increased yield by about 20% over semi-dwarf varieties (Yuan et al., 1994).
The desirable floral traits for male sterile lines studied were days to 50%
flowering, glume angle, panicle exsertion percentage, stigma exsertion percentage, pollen
sterility percentage and out crossing percentage. Therefore, the present study was
conducted with an objective to evaluate CGMS lines for floral characters at Centre for Plant
Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore.
Thirty CGMS lines of rice (Oryza sativa L.) of diverse sources were raised during
September 2007 at paddy breeding station, Tamil Nadu Agricultural University, Coimbatore. All
the lines were grown in three replications in randomized block design and a spacing
of 20x20 cm was adopted. Observations were recorded for five florets of different
plants for floral traits in all the genotypes. The traits, namely, days to 50% flowering,
glume angle, panicle exsertion percentage, stigma exsertion percentage, pollen
sterility percentage, awning, spikelet fertility percentage and out crossing percentage were
studied by routine procedure. About 10-15 spikelets from the freshly emerged panicles of all
the 12 plants were collected and examined under microscope with 1% Iodine
Potassium Iodide (I-KI) solution for pollen sterility assessment. Five panicles per plant were
evaluated for natural seed set percent. Panicles emerging from the sheath were bagged with
butter paper bags prior to anthesis to prevent cross-pollination. Bagged panicles were
harvested to assess seed setting percent. |