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  The IUP Journal of Biotechnology
Antimicrobial Activity of Six Indian Spices
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Antimicrobial activity of different extracts (ethanol, acetone, petroleum ether and aqueous) of six spices, viz., Ferula assafoetida (Asafoetida), Bunium persicum (Black cumin), Foeniculum vulgare (Fennel), Amomum subulatum (Black cardamom), Glycyrrhiza glabra (Licorice) and Nigella sativa (Black Caraway) were evaluated against seven bacterial strains (Salmonella typhimurium, Salmonella abony, Escherichia coli, Staphylococcus aureus, Clostridium butyricum, Bacillus cereus and Micrococcus luteus) and one yeast strain (Candida albicans). Among six spices tested, A. subulatum,N. sativa and G. glabra exhibited significant antimicrobial activity. The Minimum Inhibitory Concentration (MIC) of the active extracts against tested organisms ranged from 0.13 to 3.7 mg/mL for A. subulatum; 0.015 to 3.7 mg/mL for G. glabra; and 0.045 to 11.11 mg/mL for N. sativa for different microorganisms. This study suggests the selection of spices for further pharmacological investigation.

 
 

According to WHO, diarrhea worldwide kills 1.5 million children every year, which is more than the number of deaths due to AIDS (WHO, 2009). In developing countries, every year children under three years of age experience on an average three episodes of diarrhea and each episode deprives them the nutrition necessary for growth. Major reason of diarrheal deaths is enterotoxin produced by different pathogenic bacteria such as Salmonella, Escherichia, etc. (Oscar et al., 2009). Indiscriminate use of existing antimicrobial compounds has led to the emergence of resistance in microorganisms and due to which, food hygiene in food industries and hospitals are critically significant as large number of persons can be exposed to infection and related complications. As a result of several undesirable effects of chemical and physical preservatives, there is an interest in natural substances that can inhibit food spoilage microorganisms while preserving the nutritive quality and taste.

Spices are well-known for their flavoring, medicinal and antioxidant properties (Unver et al., 2009). Many spices have traditional commercial uses, such as ingredients of medicines, perfumes, incense and soaps. Active compounds of several spices have been included in class of naturally occurring food preservatives and have their inclusion in foods allowed by food production regulatory agencies (Brull and Coote, 1999). The present study evaluates antimicrobial activity of ethanol, acetone, petroleum ether and aqueous extracts of six spices that are commonly used in Indian cuisine.

 
 
 

Biotechnology Journal, Six Indian Spices, Antioxidant Properties, Food Production Regulatory Agencies, Therapeutic Drugs, Antimicrobial Activities, Food Spoiling Microorganisms, Antibacterial Activity, Industrial Microbes, Antioxidant Properties, Minimum Inhibitory Concentration.